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Journal of Molecular Cell Biology Advance Access published online on June 3, 2009
Journal of Molecular Cell Biology, doi:10.1093/jmcb/mjp003
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© The Author (2009). Published by Oxford University Press on behalf of Journal of Molecular Cell Biology, IBCB, SIBS, CAS. All rights reserved

Generation of Pig-Induced Pluripotent Stem Cells with a Drug-Inducible System

Zhao Wu1,{dagger}, Jijun Chen1,{dagger}, Jiangtao Ren1, Lei Bao1, Jing Liao1, Chun Cui1, Linjun Rao1, Hui Li2, Yijun Gu1, Huiming Dai1, Hui Zhu1, Xiaokun Teng3, Lu Cheng1 and Lei Xiao1,*

1 Laboratory of Molecular Cell Biology, Institute of Biochemistry and Cell Biology, Cell Bank, Stem Cell Bank, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, People's Republic of China
2 Xiangtan Center Hospital, Hunan 411100, People's Republic of China
3 National Engineering Center for Biochip at Shanghai, Zhangjiang Hi-Tech Park, Pudong, Shanghai 201203, People's Republic of China

* Correspondence to: Lei Xiao, Tel: +86 21 54921386; Fax: +86 21 54921388. E-mail: leixiao{at}sibs.ac.cn


  Abstract

Domesticated ungulate pluripotent embryonic stem (ES) cell lines would be useful for generating precise gene-modified animals. To date, many efforts have been made to establish domesticated ungulate pluripotent ES cells from early embryos without success. Here, we report the generation of porcine-induced pluripotent stem (iPS) cells using drug-inducible expression of defined factors. We showed that porcine iPS cells expressed alkaline phosphatase, SSEA3, SSEA4, Tra-1-60, Tra-1-81, Oct3/4, Nanog, Sox2, Rex1 and CDH1. Pig iPS cells expressed high levels of telomerase activity and showed normal karyotypes. These cells could differentiate into cell types of all three germ layers in vitro and in teratomas. Our study reveals properties of porcine pluripotent stem cells that may facilitate the eventual establishment of porcine ES cells. Moreover, the porcine iPS cells produced may be directly useful for the generation of precise gene-modified pigs.

Keywords: pluripotency, reprogram, iPS cells, pig, ungulate, embryonic stem cells

Received March 21, 2009; Revised April 11, 2009; Accepted April 14, 2009

{dagger} These authors contributed equally to this work.


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